UCSB NanoFab Microscope Training - Revision history

John d: /* Depth Measurements using focus */ added knob turning direction

2026-06-24T22:07:33Z

Depth Measurements using focus: added knob turning direction

← Older revision		Revision as of 22:07, 24 June 2026
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	Many microscopes have gradations marked on the fine-focus knob, indicating microns of movement of the sample stage. Using these marks, one can focus on different surfaces and use the gradations to estimate how many microns of vertical separation is present between the two surfaces. Measurement Error stems from your your ability to tell whether a surface is in-focus, often 5-10µm for 5x mag, and more accurate for higher magnifications.		Many microscopes have gradations marked on the fine-focus knob, indicating microns of movement of the sample stage. Using these marks, one can focus on different surfaces and use the gradations to estimate how many microns of vertical separation is present between the two surfaces. Measurement Error stems from your your ability to tell whether a surface is in-focus, often 5-10µm for 5x mag, and more accurate for higher magnifications.

	If the microscope has motorized focus, the control software may display the focus height, which can then be used to measure the depth between different focus heights.		If the microscope has motorized focus, the control software may display the focus height, which can then be used to measure the depth between different focus heights.<blockquote>'''Turning the manual focus knob ''towards you'' means the stage moves ''Down'', away from the microscope objective.''' </blockquote>

	===Focusing on a sample with no features (Focus Stop)===		===Focusing on a sample with no features (Focus Stop)===

John d: /* Back/Front-side Illumination */

2026-06-16T20:45:35Z

Back/Front-side Illumination

← Older revision		Revision as of 20:45, 16 June 2026
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	The Olympus Fluoroscope has a separate illuminator for this, so you enable Diascopic mode by simply turning on the backside illuminator. This scope therefore allows both and and back-side illumination simultaneously.		The Olympus Fluoroscope has a separate illuminator for this, so you enable Diascopic mode by simply turning on the backside illuminator. This scope therefore allows both and and back-side illumination simultaneously.

	[[File:Nikon Microscope Training - front panel Epi Dia switch.png\|alt=Photo of the Epi/Dia switch\|none\|thumb\|300x300px\|Episcopic/Diascopic switch on the Nikon Eclipse ~~microscope~~.]]<gallery widths="400" style="border: 2px;" mode="packed" heights="150" caption="Comparison of Reflective (Epi.) vs. Transmission (Dia.) for checking a through-wafer etch.">		[[File:Nikon Microscope Training - front panel Epi Dia switch.png\|alt=Photo of the Epi/Dia switch\|none\|thumb\|300x300px\|Episcopic/Diascopic switch on the Nikon Eclipse Microscope #4. ]]<gallery widths="400" style="border: 2px;" mode="packed" heights="150" caption="Comparison of Reflective (Epi.) vs. Transmission (Dia.) for checking a through-wafer etch.">
	File:Microscope Training - Epi mode example - 2022-12-21 XYZ W31A after metal --0011.jpg\|Released Membrane in Reflective/Epi mode imaging. Features are ~5-10µm. (Photo: [[Demis D. John]])		File:Microscope Training - Epi mode example - 2022-12-21 XYZ W31A after metal --0011.jpg\|Released Membrane in Reflective/Epi mode imaging. Features are ~5-10µm. (Photo: [[Demis D. John]])
	File:Microscope Training - Dia mode example - 2022-12-21 XYZ W31A after metal --0005.jpg\|Transmissive mode (Diascopic) illumination of a released membrane, confirming that the Silicon substrate has been fully removed and the membrane is free-standing. (Photo: [[Demis D. John]])		File:Microscope Training - Dia mode example - 2022-12-21 XYZ W31A after metal --0005.jpg\|Transmissive mode (Diascopic) illumination of a released membrane, confirming that the Silicon substrate has been fully removed and the membrane is free-standing. (Photo: [[Demis D. John]])

John d: /* Back/Front-side Illumination */

2026-06-16T20:45:10Z

Back/Front-side Illumination

← Older revision		Revision as of 20:45, 16 June 2026
Line 198:		Line 198:
	The Olympus Fluoroscope has a separate illuminator for this, so you enable Diascopic mode by simply turning on the backside illuminator. This scope therefore allows both and and back-side illumination simultaneously.		The Olympus Fluoroscope has a separate illuminator for this, so you enable Diascopic mode by simply turning on the backside illuminator. This scope therefore allows both and and back-side illumination simultaneously.

	[[File:Nikon Microscope Training - front panel Epi Dia switch.png\|alt=Photo of the Epi/Dia switch\|none\|thumb\|300x300px\|Episcopic/Diascopic switch on Nikon Eclipse microscope]]<gallery widths="400" style="border: 2px;" mode="packed" heights="150" caption="Comparison of Reflective (Epi.) vs. Transmission (Dia.) for checking a through-wafer etch.">		[[File:Nikon Microscope Training - front panel Epi Dia switch.png\|alt=Photo of the Epi/Dia switch\|none\|thumb\|300x300px\|Episcopic/Diascopic switch on the Nikon Eclipse microscope.]]<gallery widths="400" style="border: 2px;" mode="packed" heights="150" caption="Comparison of Reflective (Epi.) vs. Transmission (Dia.) for checking a through-wafer etch.">
	File:Microscope Training - Epi mode example - 2022-12-21 XYZ W31A after metal --0011.jpg\|Released Membrane in Reflective/Epi mode imaging. Features are ~5-10µm. (Photo: [[Demis D. John]])		File:Microscope Training - Epi mode example - 2022-12-21 XYZ W31A after metal --0011.jpg\|Released Membrane in Reflective/Epi mode imaging. Features are ~5-10µm. (Photo: [[Demis D. John]])
	File:Microscope Training - Dia mode example - 2022-12-21 XYZ W31A after metal --0005.jpg\|Transmissive mode (Diascopic) illumination of a released membrane, confirming that the Silicon substrate has been fully removed and the membrane is free-standing. (Photo: [[Demis D. John]])		File:Microscope Training - Dia mode example - 2022-12-21 XYZ W31A after metal --0005.jpg\|Transmissive mode (Diascopic) illumination of a released membrane, confirming that the Silicon substrate has been fully removed and the membrane is free-standing. (Photo: [[Demis D. John]])

John d: /* Back/Front-side Illumination */

2026-06-16T20:44:46Z

Back/Front-side Illumination

← Older revision		Revision as of 20:44, 16 June 2026
Line 198:		Line 198:
	The Olympus Fluoroscope has a separate illuminator for this, so you enable Diascopic mode by simply turning on the backside illuminator. This scope therefore allows both and and back-side illumination simultaneously.		The Olympus Fluoroscope has a separate illuminator for this, so you enable Diascopic mode by simply turning on the backside illuminator. This scope therefore allows both and and back-side illumination simultaneously.

	[[File:Nikon Microscope Training - front panel Epi Dia switch.png\|alt=Photo of the Epi/Dia switch\|none\|thumb\|300x300px\|Episcopic/Diascopic switch on Nikon Eclipse microscope]]<gallery widths="400" style="border: 2px;" caption="Comparison of Reflective (Epi.) vs. Transmission (Dia.) ~~mode imaging,~~ for checking ~~that~~ a through-wafer etch ~~is complete~~.">		[[File:Nikon Microscope Training - front panel Epi Dia switch.png\|alt=Photo of the Epi/Dia switch\|none\|thumb\|300x300px\|Episcopic/Diascopic switch on Nikon Eclipse microscope]]<gallery widths="400" style="border: 2px;" mode="packed" heights="150" caption="Comparison of Reflective (Epi.) vs. Transmission (Dia.) for checking a through-wafer etch.">
	File:Microscope Training - Epi mode example - 2022-12-21 XYZ W31A after metal --0011.jpg\|Released Membrane in Reflective/Epi mode imaging. Features are ~5-10µm. (Photo: [[Demis D. John]])		File:Microscope Training - Epi mode example - 2022-12-21 XYZ W31A after metal --0011.jpg\|Released Membrane in Reflective/Epi mode imaging. Features are ~5-10µm. (Photo: [[Demis D. John]])
	File:Microscope Training - Dia mode example - 2022-12-21 XYZ W31A after metal --0005.jpg\|~~Backside/~~Transmissive mode (Diascopic) illumination of a released membrane, confirming that the Silicon substrate has been fully removed and the membrane is free-standing. (Photo: [[Demis D. John]])		File:Microscope Training - Dia mode example - 2022-12-21 XYZ W31A after metal --0005.jpg\|Transmissive mode (Diascopic) illumination of a released membrane, confirming that the Silicon substrate has been fully removed and the membrane is free-standing. (Photo: [[Demis D. John]])
	</gallery>		</gallery>
	Written by [[Demis D. John]], 2024-11-25		Written by [[Demis D. John]], 2024-11-25

John d: /* Back/Front-side Illumination */

2026-06-16T20:41:58Z

Back/Front-side Illumination

← Older revision		Revision as of 20:41, 16 June 2026
Line 198:		Line 198:
	The Olympus Fluoroscope has a separate illuminator for this, so you enable Diascopic mode by simply turning on the backside illuminator. This scope therefore allows both and and back-side illumination simultaneously.		The Olympus Fluoroscope has a separate illuminator for this, so you enable Diascopic mode by simply turning on the backside illuminator. This scope therefore allows both and and back-side illumination simultaneously.

	[[File:Nikon Microscope Training - front panel Epi Dia switch.png\|alt=Photo of the Epi/Dia switch\|none\|thumb\|300x300px\|Episcopic/Diascopic switch on Nikon Eclipse microscope]]<gallery widths="400" caption="Comparison of Reflective (Epi.) vs. Transmission (Dia.) mode imaging, for checking that a through-wafer etch is complete.">		[[File:Nikon Microscope Training - front panel Epi Dia switch.png\|alt=Photo of the Epi/Dia switch\|none\|thumb\|300x300px\|Episcopic/Diascopic switch on Nikon Eclipse microscope]]<gallery widths="400" style="border: 2px;" caption="Comparison of Reflective (Epi.) vs. Transmission (Dia.) mode imaging, for checking that a through-wafer etch is complete.">
	File:Microscope Training - Epi mode example - 2022-12-21 XYZ W31A after metal --0011.jpg\|Released Membrane in Reflective/Epi mode imaging. Features are ~5-10µm. (Photo: [[Demis D. John]])		File:Microscope Training - Epi mode example - 2022-12-21 XYZ W31A after metal --0011.jpg\|Released Membrane in Reflective/Epi mode imaging. Features are ~5-10µm. (Photo: [[Demis D. John]])
	File:Microscope Training - Dia mode example - 2022-12-21 XYZ W31A after metal --0005.jpg\|Backside/Transmissive mode (Diascopic) illumination of a released membrane, confirming that the Silicon substrate has been fully removed and the membrane is free-standing. (Photo: [[Demis D. John]])		File:Microscope Training - Dia mode example - 2022-12-21 XYZ W31A after metal --0005.jpg\|Backside/Transmissive mode (Diascopic) illumination of a released membrane, confirming that the Silicon substrate has been fully removed and the membrane is free-standing. (Photo: [[Demis D. John]])

John d: /* Back/Front-side Illumination */ reorg

2026-06-16T20:40:53Z

Back/Front-side Illumination: reorg

← Older revision		Revision as of 20:40, 16 June 2026
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	=== Back/Front-side Illumination ===		=== Back/Front-side Illumination ===

	* "'''Episcopic'''" illumination ~~means top-side illumination, that is '''reflective'''-~~mode ~~imaging (light is reflecting off the top surface).~~		* "'''Episcopic'''" illumination mode
			** Top-side illumination, that is '''reflective'''-mode imaging (light is reflecting off the top surface).
	** '''Please set back to "Epi" (reflective) mode when you are done.'''		** '''Please set back to "Epi" (reflective) mode when you are done.'''
	* "'''Diascopic'''" illumination ~~means back-side illumination, that is '''transmission'''-~~mode ~~imaging (light is transmitting from the back to front of your sample).~~		* "'''Diascopic'''" illumination mode
			** Back-side illumination, that is '''transmission'''-mode imaging (light is transmitting from the back to top of your sample).
	Only microscopes with a transparent Glass stage can support Diascopic mode illumination.		** Only microscopes with a transparent Glass stage can support Diascopic mode illumination.

	The Nikon scopes use a single illuminator for this, so only one brightness control		The Nikon scopes use a single illuminator for this, so only one brightness control knob, fast to switch between each mode.

	The Olympus Fluoroscope has a separate illuminator for this, so you enable Diascopic mode by simply turning on the backside illuminator. This scope therefore allows both and and back-side illumination simultaneously.		The Olympus Fluoroscope has a separate illuminator for this, so you enable Diascopic mode by simply turning on the backside illuminator. This scope therefore allows both and and back-side illumination simultaneously.

John d: /* Back/Front-side Illumination */

2026-06-16T20:39:40Z

Back/Front-side Illumination

← Older revision		Revision as of 20:39, 16 June 2026
Line 191:		Line 191:
	** '''Please set back to "Epi" (reflective) mode when you are done.'''		** '''Please set back to "Epi" (reflective) mode when you are done.'''
	* "'''Diascopic'''" illumination means back-side illumination, that is '''transmission'''-mode imaging (light is transmitting from the back to front of your sample).		* "'''Diascopic'''" illumination means back-side illumination, that is '''transmission'''-mode imaging (light is transmitting from the back to front of your sample).
	Only ~~microscope~~ with a transparent Glass stage can support Diascopic mode illumination.		Only microscopes with a transparent Glass stage can support Diascopic mode illumination.

	The Nikon scopes use a single illuminator for this, so only one brightness control		The Nikon scopes use a single illuminator for this, so only one brightness control

John d: /* Back/Front-side Illumination */ added Olympus Fluorscope illumination metho, glass stage

2026-06-16T20:39:25Z

Back/Front-side Illumination: added Olympus Fluorscope illumination metho, glass stage

← Older revision		Revision as of 20:39, 16 June 2026
Line 188:		Line 188:
	=== Back/Front-side Illumination ===		=== Back/Front-side Illumination ===

	* "Episcopic" illumination means top-side illumination, that is reflective-mode imaging (light is reflecting off the top surface).		* "'''Episcopic'''" illumination means top-side illumination, that is '''reflective'''-mode imaging (light is reflecting off the top surface).
	** '''Please set back to "Epi" (reflective) mode when you are done.'''		** '''Please set back to "Epi" (reflective) mode when you are done.'''
	* "Diascopic" illumination means back-side illumination, that is transmission-mode imaging (light is transmitting from the back to front of your sample).		* "'''Diascopic'''" illumination means back-side illumination, that is '''transmission'''-mode imaging (light is transmitting from the back to front of your sample).
			Only microscope with a transparent Glass stage can support Diascopic mode illumination.

			The Nikon scopes use a single illuminator for this, so only one brightness control

			The Olympus Fluoroscope has a separate illuminator for this, so you enable Diascopic mode by simply turning on the backside illuminator. This scope therefore allows both and and back-side illumination simultaneously.

	[[File:Nikon Microscope Training - front panel Epi Dia switch.png\|alt=Photo of the Epi/Dia switch\|none\|thumb\|300x300px\|Episcopic/Diascopic switch on Nikon Eclipse microscope]]<gallery widths="400" caption="Comparison of Reflective (Epi.) vs. Transmission (Dia.) mode imaging, for checking that a through-wafer etch is complete.">		[[File:Nikon Microscope Training - front panel Epi Dia switch.png\|alt=Photo of the Epi/Dia switch\|none\|thumb\|300x300px\|Episcopic/Diascopic switch on Nikon Eclipse microscope]]<gallery widths="400" caption="Comparison of Reflective (Epi.) vs. Transmission (Dia.) mode imaging, for checking that a through-wafer etch is complete.">

John d: /* Back/Front-side Illumination */ set back to epi when done

2026-06-16T20:36:31Z

Back/Front-side Illumination: set back to epi when done

← Older revision		Revision as of 20:36, 16 June 2026
Line 189:		Line 189:

	* "Episcopic" illumination means top-side illumination, that is reflective-mode imaging (light is reflecting off the top surface).		* "Episcopic" illumination means top-side illumination, that is reflective-mode imaging (light is reflecting off the top surface).
	** Please set back to "~~'''~~Epi~~'''~~" (reflective) mode when you are done.		** '''Please set back to "Epi" (reflective) mode when you are done.'''
	* "Diascopic" illumination means back-side illumination, that is transmission-mode imaging (light is transmitting from the back to front of your sample).		* "Diascopic" illumination means back-side illumination, that is transmission-mode imaging (light is transmitting from the back to front of your sample).

John d: /* Back/Front-side Illumination */

2026-06-16T20:27:25Z

Back/Front-side Illumination

← Older revision		Revision as of 20:27, 16 June 2026
Line 192:		Line 192:
	* "Diascopic" illumination means back-side illumination, that is transmission-mode imaging (light is transmitting from the back to front of your sample).		* "Diascopic" illumination means back-side illumination, that is transmission-mode imaging (light is transmitting from the back to front of your sample).

	[[File:Nikon Microscope Training - front panel Epi Dia switch.png\|alt=Photo of the Epi/Dia switch\|none\|thumb\|300x300px\|Episcopic/Diascopic switch on Nikon Eclipse microscope]]		[[File:Nikon Microscope Training - front panel Epi Dia switch.png\|alt=Photo of the Epi/Dia switch\|none\|thumb\|300x300px\|Episcopic/Diascopic switch on Nikon Eclipse microscope]]<gallery widths="400" caption="Comparison of Reflective (Epi.) vs. Transmission (Dia.) mode imaging, for checking that a through-wafer etch is complete.">
	[[File:Microscope Training - Epi mode example - 2022-12-21 XYZ W31A after metal --0011.jpg~~\|alt=photo of epi mode illumination\|none\|thumb~~\|Released Membrane Reflective/Epi mode imaging ~~example~~ (Photo: Demis D. John)]]		File:Microscope Training - Epi mode example - 2022-12-21 XYZ W31A after metal --0011.jpg\|Released Membrane in Reflective/Epi mode imaging. Features are ~5-10µm. (Photo: [[Demis D. John]])
	[[File:Microscope Training - Dia mode example - 2022-12-21 XYZ W31A after metal --0005.jpg~~\|alt=photo of diascopic illuminated mambrane\|none\|thumb~~\|Backside/Transmissive mode (Diascopic) illumination of a released membrane, confirming that the Silicon substrate has been fully removed and the membrane is free-standing. ~~Features~~ ~~are ~5-10µm~~.]]		File:Microscope Training - Dia mode example - 2022-12-21 XYZ W31A after metal --0005.jpg\|Backside/Transmissive mode (Diascopic) illumination of a released membrane, confirming that the Silicon substrate has been fully removed and the membrane is free-standing. (Photo: [[Demis D. John]])
			</gallery>



	Written by [[Demis D. John]], 2024-11-25		Written by [[Demis D. John]], 2024-11-25