Fluorescence Microscope (Olympus MX51): Difference between revisions
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|picture=Fluorescence.jpg |
|picture=Fluorescence.jpg |
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|type = Inspection, Test and Characterization |
|type = Inspection, Test and Characterization |
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|super= |
|super= Demis D. John |
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|location=Bay 6 |
|location=Bay 6 |
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|description = |
|description = Optical Microscope |
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|manufacturer = Olympus |
|manufacturer = Olympus |
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|model = MX51 |
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|materials = |
|materials = |
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|toolid= |
|toolid= |
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}} |
}} |
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=About= |
=About= |
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System for general purpose imaging. The system has bright field, dark-field, DIC imaging mode and Three fluorescence filters. Screen captures and calibrated measurements can be made through use of native Olympus' Streams Essential Software, installed on the attached computer. |
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=Detailed Specifications= |
=Detailed Specifications= |
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* Native ''Olympus Streams Essentials'' software & Camera: |
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** Digital image capture & calibrated measurement (calibrations locked) |
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* Objectives: 5x, 10x, 20x, 50x, 100x, 150x |
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* Reflected (Episcopic) & Transmitted (Diascopic) Illumination modes |
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* Filters via rotatable selector: |
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** '''BF''': Bright Field normal top-illuminated imaging |
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** '''DF''': Dark-Field imaging |
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** '''DIC''': Differential Interference Contrast "Nomarski" Imaging. Also requires "DIC-R" prism to be inserted. Polarization manually selected on DIC-R prism. |
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* Fluorescence Filters (see [https://www.olympus-lifescience.com/en/microscope-resource/primer/techniques/fluorescence/anatomy/fluoromicroanatomy/ Olympus tutorial]): |
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** '''''You must contact Tony Bosch for training on Fluorescence before using this feature!!!''''' |
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** '''WB''': "Blue" Fluorescence Filter. Excitation: 450-480nm / Dichroic 500nm / Barrier 515nm+ |
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** '''WG''': "Green" Fluorescence Filter. Excitation: 510-550nm / Dichroic 570nm / Barrier 590nm+ |
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** '''WU''': "Ultraviolet" Fluorescence Filter. Excitation: 330-385nm / Dichroic 400nm / Barrier 420nm+ |
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=Documentation= |
=Documentation= |
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[[media:Olympus-Stream-Software.pdf|Stream Imaging Software Manual]] |
*[[media:Olympus-Stream-Software.pdf|Stream Imaging Software Manual]] |
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Latest revision as of 18:09, 15 April 2020
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About
System for general purpose imaging. The system has bright field, dark-field, DIC imaging mode and Three fluorescence filters. Screen captures and calibrated measurements can be made through use of native Olympus' Streams Essential Software, installed on the attached computer.
Detailed Specifications
- Native Olympus Streams Essentials software & Camera:
- Digital image capture & calibrated measurement (calibrations locked)
- Objectives: 5x, 10x, 20x, 50x, 100x, 150x
- Reflected (Episcopic) & Transmitted (Diascopic) Illumination modes
- Filters via rotatable selector:
- BF: Bright Field normal top-illuminated imaging
- DF: Dark-Field imaging
- DIC: Differential Interference Contrast "Nomarski" Imaging. Also requires "DIC-R" prism to be inserted. Polarization manually selected on DIC-R prism.
- Fluorescence Filters (see Olympus tutorial):
- You must contact Tony Bosch for training on Fluorescence before using this feature!!!
- WB: "Blue" Fluorescence Filter. Excitation: 450-480nm / Dichroic 500nm / Barrier 515nm+
- WG: "Green" Fluorescence Filter. Excitation: 510-550nm / Dichroic 570nm / Barrier 590nm+
- WU: "Ultraviolet" Fluorescence Filter. Excitation: 330-385nm / Dichroic 400nm / Barrier 420nm+