Fluorescence Microscope (Olympus MX51)

Fluorescence Microscope (Olympus MX51) Tool Type Inspection, Test and Characterization Location Bay 6 Supervisor Tony Bosch Supervisor Phone (805) 893-3486 Supervisor E-Mail bosch@ece.ucsb.edu Description Manufacturer Olympus

About

System for general purpose imaging. The system has bright field, dark-field, and DIC imaging modes. Screen captures and measurements on samples can be made through use of Olympus' Streams Essential Software, installed on the attached computer.

Detailed Specifications

• Digital image capture & calibrated measurement via integrated Olympus Streams Essentials software.
• Objectives: 5x, 10x, 20x, 50x, 100x, 150x
• Reflected (Episcopic) & Transmitted (Diascopic) Illumination modes
• Filters via rotatable selector:
  • BF: Bright Field normal top-illuminated imaging
  • DF: Dark-Field imaging
  • DIC: Differential Interference Contrast "Nomarski" Imaging. Also requires "DIC-R" prism to be inserted. Polarization manually selected on DIC-R prism.
• Fluorescence Filters (see Olympus tutorial):
  • You must contact Tony Bosch for training on Fluorescence before using this feature!!!
  • WB: "Blue" Fluorescence Filter. Excitation: 450-480nm / Dichroic 500nm / Barrier 515nm+
  • WG: "Green" Fluorescence Filter. Excitation: 510-550nm / Dichroic 570nm / Barrier 590nm+
  • WU: "Ultraviolet" Fluorescence Filter. Excitation: 330-385nm / Dichroic 400nm / Barrier 420nm+

Documentation

• Stream Imaging Software Manual