Digital Microscope (Olympus DSX1000): Difference between revisions
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|picture= |
|picture=OlympusDSX1000.jpg |
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|type = Inspection, Test and Characterization |
|type = Inspection, Test and Characterization |
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|super= Tony Bosch |
|super= Tony Bosch |
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|super2= Demis D. John |
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|phone=(805)839-3918x217 |
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|location=Bay 4 |
|location=Bay 4 |
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|email=bosch@ece.ucsb.edu |
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|materials = |
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=About= |
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The Olympus DSX1000 is a high-resolution digital microscope with full electronic control, multiple imaging modes and sophisticated analysis software built-in. |
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The LEXT OLS4000 3D Laser Measuring Microscope is designed for nanometer level imaging, 3D measurement and roughness measurement. Magnification ranges from 108x - 17,280x satisfy the needs of today's researchers. For a complete description of the tool and its capabilities, please see the above link to the manufacturer’s website. |
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The built-in optical magnification allows for high-resolution imaging without changing objectives. The system has applies Digital image stabilization and software image enhancement. |
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[[File:Olypmus LEXT Example Profile Measurement.png|alt=Measurement of 5µm wide posts|thumb|205x205px|Example Height/Profile Measurement (click to enlarge)]] |
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The LEXT allows you to take height measurements of features too small to reach with a physical Stylus needle, in a non-contact mode that is much faster than engaging an AFM tip. In addition, very large aspect-ratios can be measured - for example, 1mm depth x 50µm width. Confocal microscopy ensures that only surfaces that are in-focus will return a signal to the microscope detector. The LEXT sweeps the focus motor and captures a 2D scan of your sample at each focus step, taken with a blue laser (405 nm). After the scan, the height of each surface is calculated by the focus step which produced the highest laser intensity, and this is constructed into a 3D image by the OLS software. |
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Users must be <u>very careful</u> to '''avoid crashing the 50x objective''', because it does not rotate out of the way like our other microscopes. |
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Because this is an optical method, films that are optically transparent to blue light and/or have transparent sloping sidewalls can produce non-physical measurements due to optical interference on the sample. In addition, features on the order of the laser wavelength can produce unreliable/non-physical data - for example, the data near (within ~500 nm) very steep slopes, or features ≤ 1.0 µm wide may not be reliable. |
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Surfaces that are opaque to the laser wavelength work best, although measurements on rectangular transparent edges do work relatively well. Edges of steps where light does not reflect in an ideal manner often produce non-physical features, typically manifesting as trenches/spikes right next to the sidewall. |
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Digital only (view through computer) |
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Optical Magnification ?? |
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Technically the height-resolution is specified as 10 nm (the height resolution of the focus motor), but in practice, noise and optical uncertainties worsen this spec. |
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Objectives: 5x, 50x |
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Imaging Modes: Bright Field, Dark Field, DIC (Nomarski), Oblique Illumination (varying angle), Polarized Illumination (varying Pol.) |
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[https://drive.google.com/drive/folders/1NsTb1UYDkTTvWU2AjfujuqYbKstos3W7?usp=sharing LEXT OLS4000 Offline Software] |
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Top Reflected Illumination (Episcopic) |
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Camera Capture: ??? MP |
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[[Olympus Stream Desktop Offline Software (TEMP)]] |
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== Other Info == |
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'''''Equip > Characterization > <u>[[Microscopes]]</u>''''' - ''see a list of all our microscopes'' |
Latest revision as of 18:49, 30 August 2022
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About
The Olympus DSX1000 is a high-resolution digital microscope with full electronic control, multiple imaging modes and sophisticated analysis software built-in.
The built-in optical magnification allows for high-resolution imaging without changing objectives. The system has applies Digital image stabilization and software image enhancement.
Users must be very careful to avoid crashing the 50x objective, because it does not rotate out of the way like our other microscopes.
Technique & Capabilities
Digital only (view through computer)
Optical Magnification ??
Objectives: 5x, 50x
Imaging Modes: Bright Field, Dark Field, DIC (Nomarski), Oblique Illumination (varying angle), Polarized Illumination (varying Pol.)
Top Reflected Illumination (Episcopic)
Camera Capture: ??? MP
Operating Procedures
Olympus DSX1000 Quick Start.pdf
Offline software
Olympus Stream Desktop Offline Software (TEMP)
Other Info
Equip > Characterization > Microscopes - see a list of all our microscopes