Microscopes: Difference between revisions

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|picture=microscopes.jpg
|picture=microscopes.jpg
|type = Inspection, Test and Characterization
|type = Inspection, Test and Characterization
|super= Tony Bosch
|super= Demis D. John
|location=Bay 3, 6 & 7
|location=Bay 3, 6 & 7
|description = Optical Microscopes
|description = Optical Microscopes
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}}
}}


== Procedures & Tools ==
==Procedures & Tools==
Most General-Use Microscopes do not require formal training - please see the pages below for specifics.

===[[UCSB NanoFab Microscope Training|'''Microscope Training Guide''']]===
===[[UCSB NanoFab Microscope Training|'''Microscope Training Guide''']]===
Use the guide linked above for general usage info & the many advanced features available on most of our microscopes. New users are encouraged to read this, and also request in-person training by [[Demis D. John|Demis]].
This guide explains general usage info & the many advanced features available on most of our microscopes. New users are encouraged to read this, and optionally request in-person training by [[Demis D. John|Demis]].


Includes topics like:
⚫
=== Image Analysis Software ===

*Basic usage
*Preventing a crash
⚫
*Bright-Field/Dark Field
*How to dis/enable DIC/Nomarskiimaging

<ins>'''DO NOT TURN MICROSCOPE OBJECTIVES BY HAND''' for motorized microscopes! Most of our scopes have motorized objective turrets, use the electronic buttons instead.</ins>

⚫
===Image Analysis Software===
Many of our microscopes have cameras and software for image capture. If you know the ''microscope and objective'' used for acquiring a photo, you can make calibrated measurements on the photos at your own desktop using the following software:
Many of our microscopes have cameras and software for image capture. If you know the ''microscope and objective'' used for acquiring a photo, you can make calibrated measurements on the photos at your own desktop using the following software:

*[https://www.amscope.com/software-download AmScope Software] - microscope image analysis software
*[https://www.amscope.com/software-download AmScope Software] - free microscope image analysis software
**[[Measurements and Imaging with Amscope Camera - Quickstart Usage Guide]]
**AmScope Calibration File containing calibrations for all NanoFab microscopes: [https://wiki.nanotech.ucsb.edu/wiki/Images/uploads/2020/AmScopeCalsAll.magn Download Here]
**AmScope Calibration File containing calibrations for all NanoFab microscopes: [https://wiki.nanotech.ucsb.edu/wiki/Images/uploads/2020/AmScopeCalsAll.magn Download Here]
**Also available on '''''Nanofiles-SFTP / Manuals / Amscope'''''
**Also available on '''''Nanofiles-SFTP / Manuals / Amscope'''''
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**There are many [https://imagej.net/Category:Plugins other useful plugins], for particle counting, [https://stackoverflow.com/questions/6230353/how-to-create-gif-animation-from-a-stack-of-jpgs/37193012#37193012 creating animations] etc.
**There are many [https://imagej.net/Category:Plugins other useful plugins], for particle counting, [https://stackoverflow.com/questions/6230353/how-to-create-gif-animation-from-a-stack-of-jpgs/37193012#37193012 creating animations] etc.


==General-Use Microscopes==
==Nikon==
''These microscopes do not require formal training to use. However, if you have never used a motorized objective turret, please see the [[UCSB NanoFab Microscope Training|'''Microscope Training Guide''']] for safety info.''
The Nikon scopes have 3rd party cameras that are connected to computers via AM Scope software which allows users to digitally capture images through the microscope and make calibrated measurements.


===Microscope #2: Nikon Optiphot 200 (Bay 7)===
===Microscope #2: Nikon Optiphot 200 (Bay 2)===


*Trinocular: Ocular Binoc. + Camera (Simultaneous)
*Trinocular: Ocular Binoc. + Camera (Simultaneous)
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**DIC only available on 100x and 150x mags due to image quality reduction at low mags due to DIC prisms which were removed for low mags.
**DIC only available on 100x and 150x mags due to image quality reduction at low mags due to DIC prisms which were removed for low mags.
*Top Reflected Illumination (Episcopic)
*Top Reflected Illumination (Episcopic)
*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here].
*AMScope 14MP Cameras (Model MU1400B) with calibrated software for measurements. Free Software available for calibrated measurements. [[Microscopes#Image Analysis Software|See above]] for software & calibration file link.


===Microscope #3: Nikon Eclipse L200 (Bay 6)===
===Microscope #3: Nikon Eclipse L200 (Bay 6)===
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*Differential Interference Contrast (DIC/Nomarski)
*Differential Interference Contrast (DIC/Nomarski)
*Top Reflected Illumination (Episcopic)
*Top Reflected Illumination (Episcopic)
*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here].
*AMScope 14MP Cameras (Model MU1400B) with calibrated software for measurements. Free Software available for calibrated measurements. [[Microscopes#Image Analysis Software|See above]] for software & calibration file link.


===Microscope #4: Nikon Eclipse L200D (Bay 6)===
===Microscope #4: Nikon Eclipse L200D (Bay 6)===
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*Differential Interference Contrast (DIC/Nomarski)
*Differential Interference Contrast (DIC/Nomarski)
*Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
*Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here].
*AMScope 14MP Cameras (Model MU1400B) with calibrated software for measurements. Free Software available for calibrated measurements. [[Microscopes#Image Analysis Software|See above]] for software & calibration file link.

==Olympus==

The lab has two general purpose Olympus microscopes, and two specialized microscopes that require training to use, although both of these can also be used for general imaging.
⚫
[[File:Microscope 01 Olympus BHMJL crop.png|alt=Photo of Microscope #01 in the Characterization lab, Room 1111|thumb|200x200px|Microscope #01 in the Characterization lab, Room 1111]]

⚫
===Microscope #1: Olympus BHMJL (Char. Lab, Room 1111)===

⚫
*Trinocular: Ocular Binoc. + Camera (Exclusive)
⚫
*LED Illuminator, Variable
⚫
*Objectives: ''10x, 20x, 50x, 100x''
⚫
*Bright/Dark Field
⚫
*Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
⚫
*AMScope 14MP Cameras with calibrated software for measurements. Software available for offline measurements: [https://www.amscope.com/software-download Link here].
*User Manual: [https://wiki.nanotech.ucsb.edu/wiki/index.php/File:Olympus_-_BHM_Microscope_-_Instruction_Manual.pdf Olympus BHM Microscopes - Instruction Manual (PDF)]


===Microscope #5: [[Fluorescence Microscope (Olympus MX51)|Fluorescence Microscope: Olympus MX51 (Bay 6)]]===
===Microscope #5: [[Fluorescence Microscope (Olympus MX51)|Fluorescence Microscope: Olympus MX51 (Bay 6)]]===
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*Three Fluorescence Filters (requires training, see main tool page for specs.)
*Three Fluorescence Filters (requires training, see main tool page for specs.)


⚫
[[File:Microscope 01 Olympus BHMJL crop.png|alt=Photo of Microscope #01 in the Characterization lab, Room 1111|thumb|200x200px|Microscope #01]]
⚫
===Microscope #6: [[Deep UV Optical Microscope (Olympus)|DUV Microscope: Olympus MX61A-DUV (Bay 7)]]===

⚫
===Microscope #1: Olympus BHMJL (Room 1111)===

⚫
*Trinocular: Ocular Binoc. + Camera (Exclusive)
⚫
*LED Illuminator, Variable
⚫
*Objectives: ''10x, 20x, 50x, 100x''
*Bright/Dark Field
⚫
*Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
⚫
*AMScope 14MP Cameras (Model MU1400B) with calibrated software for measurements. Free Software available for calibrated measurements. [[Microscopes#Image Analysis Software|See above]] for software & calibration file link.

===Microscope #8: AmScope Wide Field of View Stereoscope (Bay 4)===
[[File:Amscope Stereoscope Photo.jpg|alt=photo of the Amscope Sterescope microscope in bay 4|thumb|250x250px|Microscope #8: Amscope Stereoscope]]

*Integrated camera for taking large field of view pictures. 
*The field of view is about 25mm in '''Y''' and larger in '''X''' at the lowest magnification. 
*Nanofiles Sync for file transfer: FTP folder name "''amscope''". 
*AMScope software on the laptop to use the camera.
*On the side of the main scope there is a small lever that is used to divert the image from the right eyepiece to the camera for imaging, the left eyepiece remains open for viewing at all times. 

==Microscopes Requiring Training==
''The following microscopes require training from the supervisor. Click on the appropriate tool page to see the supervisor info.''

⚫
===Microscope #6: [[Deep UV Optical Microscope (Olympus)|DUV Microscope: Olympus MX61A-DUV (Bay 4)]]===
Please see the main tool page for detailed info on this microscope: [[Deep UV Optical Microscope (Olympus)]]
Please see the main tool page for detailed info on this microscope: [[Deep UV Optical Microscope (Olympus)]]
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.

'''DUV Camera is non-operational and unable to be repaired. Visible camera is still functional. 2022-02'''


*Motorized Stage + Objective Turret
*Motorized Stage + Objective Turret
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**DUV-100x sub-micron imaging/measurement
**DUV-100x sub-micron imaging/measurement


==[[Laser Scanning Confocal M-scope (Olympus LEXT)|Olympus LEXT Confocal Microscope (Bay 4)]]==
===Microscope #7: [[Digital Microscope (Olympus DSX1000)|Olympus DSX1000 Digital Microscope (Bay 4)]]===
Please see the main tool page for detailed info on this microscope, click the link above.
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Supervisor to get training.

*Motorized Stage + Optics
*Optical magnification via motorized optics, increases mag beyond objective’s value.
*Manual switch between 2 objectives.
*Digital Viewing via Computer
*Objectives: High-Res.: 5x, 50x // Long-Distance + Angled Viewing: 10x, 40x
*Imaging Modes:
**Bright Field
**Dark Field
**DIC/Nomarski
**Oblique Ilumination w/ variable illumination angle
**Polarized imaging w/ variable polarization
*Native Olympus Software control & Camera
**Calibrated measurements (calibrations locked)
**Z (focus) measurement via motorized stage height
**3D height Meas (for taller features)
**Rapidly capture multiple imaging modes
**Offline Analysis Software available for free
*Tilted Imaging capabilities, for deep (> few micron) features.

===[[Laser Scanning Confocal M-scope (Olympus LEXT)|Olympus LEXT Confocal Microscope (Bay 4)]]===
See the main tool page for complete info: [[Laser Scanning Confocal M-scope (Olympus LEXT)]]
See the main tool page for complete info: [[Laser Scanning Confocal M-scope (Olympus LEXT)]]
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
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**Surface roughness estimations (large roughness)
**Surface roughness estimations (large roughness)
**Thin-Film Film-Thickness Measurements (thicker films)
**Thin-Film Film-Thickness Measurements (thicker films)

===[[Filmetrics F40-UV Microscope-Mounted|Filmetrics F40-UV / Olympus BHMJL (Bay 4)]]===

*Very simple, manual microscope.
*Has spectroscopic reflectometer attached for thin-film measurements in small (<100µm) area.
*See the tool page for Training on the Filmetrics Thin-Film Measurement tool.


==Procedures & Documentation==
==Procedures & Documentation==

Latest revision as of 00:02, 2 November 2023

Microscopes
Microscopes.jpg
Tool Type Inspection, Test and Characterization
Location Bay 3, 6 & 7
Supervisor Demis D. John
Supervisor Phone (805) 893-5934
Supervisor E-Mail demis@ucsb.edu
Description Optical Microscopes
Manufacturer Nikon & Olympus



Procedures & Tools

Most General-Use Microscopes do not require formal training - please see the pages below for specifics.

Microscope Training Guide

This guide explains general usage info & the many advanced features available on most of our microscopes. New users are encouraged to read this, and optionally request in-person training by Demis.

Includes topics like:

  • Basic usage
  • Preventing a crash
  • Bright-Field/Dark Field
  • How to dis/enable DIC/Nomarskiimaging
DO NOT TURN MICROSCOPE OBJECTIVES BY HAND for motorized microscopes!  Most of our scopes have motorized objective turrets, use the electronic buttons instead.

Image Analysis Software

Many of our microscopes have cameras and software for image capture. If you know the microscope and objective used for acquiring a photo, you can make calibrated measurements on the photos at your own desktop using the following software:

General-Use Microscopes

These microscopes do not require formal training to use. However, if you have never used a motorized objective turret, please see the Microscope Training Guide for safety info.

Microscope #2: Nikon Optiphot 200 (Bay 2)

  • Trinocular: Ocular Binoc. + Camera (Simultaneous)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
    • DIC only available on 100x and 150x mags due to image quality reduction at low mags due to DIC prisms which were removed for low mags.
  • Top Reflected Illumination (Episcopic)
  • AMScope 14MP Cameras (Model MU1400B) with calibrated software for measurements. Free Software available for calibrated measurements. See above for software & calibration file link.

Microscope #3: Nikon Eclipse L200 (Bay 6)

  • Manual for Nikon Eclipse L200D
  • Trinocular: Binoc. + Camera (Mutually Exclusive)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
  • Top Reflected Illumination (Episcopic)
  • AMScope 14MP Cameras (Model MU1400B) with calibrated software for measurements. Free Software available for calibrated measurements. See above for software & calibration file link.

Microscope #4: Nikon Eclipse L200D (Bay 6)

  • Trinocular: Binoc. + Camera (Mutually Exclusive)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
  • Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
  • AMScope 14MP Cameras (Model MU1400B) with calibrated software for measurements. Free Software available for calibrated measurements. See above for software & calibration file link.

Microscope #5: Fluorescence Microscope: Olympus MX51 (Bay 6)

  • See the wiki page for the Olympus MX51 for full details
  • Trinocular: Binoc. + Camera (Simultaneous)
  • Native Olympus Stream Software:
    • Photo/video capture
    • Calibrated measurement (calibrations locked)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
  • Top (Episcopic) & Bottom (Diascopic) Illumination
  • Three Fluorescence Filters (requires training, see main tool page for specs.)
Photo of Microscope #01 in the Characterization lab, Room 1111
Microscope #01

Microscope #1: Olympus BHMJL (Room 1111)

  • Trinocular: Ocular Binoc. + Camera (Exclusive)
  • LED Illuminator, Variable
  • Objectives: 10x, 20x, 50x, 100x
  • Bright/Dark Field
  • Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
  • AMScope 14MP Cameras (Model MU1400B) with calibrated software for measurements. Free Software available for calibrated measurements. See above for software & calibration file link.

Microscope #8: AmScope Wide Field of View Stereoscope (Bay 4)

photo of the Amscope Sterescope microscope in bay 4
Microscope #8: Amscope Stereoscope
  • Integrated camera for taking large field of view pictures. 
  • The field of view is about 25mm in Y and larger in X at the lowest magnification. 
  • Nanofiles Sync for file transfer: FTP folder name "amscope". 
  • AMScope software on the laptop to use the camera.
  • On the side of the main scope there is a small lever that is used to divert the image from the right eyepiece to the camera for imaging, the left eyepiece remains open for viewing at all times. 

Microscopes Requiring Training

The following microscopes require training from the supervisor. Click on the appropriate tool page to see the supervisor info.

Microscope #6: DUV Microscope: Olympus MX61A-DUV (Bay 4)

Please see the main tool page for detailed info on this microscope: Deep UV Optical Microscope (Olympus) 

YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.

DUV Camera is non-operational and unable to be repaired. Visible camera is still functional. 2022-02
  • Motorized Stage + Objective Turret
  • Trinocular: Binoc. + Camera (Simultaneous)
  • Objectives: 5x, 10x, 20, 50x, 100x, DUV-100x
  • Filters: to be added
  • Native Olympus MX61 Software control & Camera
    • Calibrated measurements (calibrations locked)
    • Z (focus) measurement via motorized stage height
  • Deep-UV Light source + DUV Camera
    • DUV-100x sub-micron imaging/measurement

Microscope #7: Olympus DSX1000 Digital Microscope (Bay 4)

Please see the main tool page for detailed info on this microscope, click the link above. 

YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Supervisor to get training.
  • Motorized Stage + Optics
  • Optical magnification via motorized optics, increases mag beyond objective’s value.
  • Manual switch between 2 objectives.
  • Digital Viewing via Computer
  • Objectives: High-Res.: 5x, 50x // Long-Distance + Angled Viewing: 10x, 40x
  • Imaging Modes:
    • Bright Field
    • Dark Field
    • DIC/Nomarski
    • Oblique Ilumination w/ variable illumination angle
    • Polarized imaging w/ variable polarization
  • Native Olympus Software control & Camera
    • Calibrated measurements (calibrations locked)
    • Z (focus) measurement via motorized stage height
    • 3D height Meas (for taller features)
    • Rapidly capture multiple imaging modes
    • Offline Analysis Software available for free
  • Tilted Imaging capabilities, for deep (> few micron) features.

Olympus LEXT Confocal Microscope (Bay 4)

See the main tool page for complete info: Laser Scanning Confocal M-scope (Olympus LEXT) 

YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
  • Motorized stage + Objective Turret
  • 100mm wafer stage
  • Native Olympus OLS2000 Software & Built-In Camera:
    • Calibrated measurement (calibrations locked)
    • Image stitching capabilities
  • 3D Laser-Scanning Confocal Microscopy capability:
    • 3D Topographical measurement (optical profilometry)
    • Surface roughness estimations (large roughness)
    • Thin-Film Film-Thickness Measurements (thicker films)

Filmetrics F40-UV / Olympus BHMJL (Bay 4)

  • Very simple, manual microscope.
  • Has spectroscopic reflectometer attached for thin-film measurements in small (<100µm) area.
  • See the tool page for Training on the Filmetrics Thin-Film Measurement tool.

Procedures & Documentation

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