Microscopes: Difference between revisions
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|description = Optical Microscopes |
|description = Optical Microscopes |
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|manufacturer = Nikon & Olympus |
|manufacturer = Nikon & Olympus |
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}}[[UCSB NanoFab Microscope Training|'''Microscope Training''']] - Use this guide for general usage info & the many advanced features available on most of our microscopes. |
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}} |
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==Nikon== |
==Nikon== |
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The Nikon scopes have 3rd party cameras that are connected to computers via AM Scope software which allows users to digitally capture images through the microscope and make calibrated measurements. |
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The lab currently has 3 Nikon Microscopes (Microscopes #2, #3 & #4). These are located in bays 6 and 7. |
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===Microscope #1: Nikon Optiphot-2 (Char. Lab, Room 1111)=== |
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The scopes have 3rd party cameras that are connected to computers via AM Scope software which allows users to digitally capture images through the microscope and make calibrated measurements. |
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*Trinocular: Ocular Binoc. + Camera (Exclusive) |
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Calibrations versus mag are indicated in the table below for all scopes. |
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*LED Illuminator, Variable |
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*Objectives: ''Info to be added'' |
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*Bright/Dark Field |
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*Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination |
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*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here]. |
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*User Manual: [https://www.nanotech.ucsb.edu/wiki/images/2/28/Nikon-Microscope-OptiPhot-Manual.pdf Nikon Optiphot-2 (PDF)] |
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=== |
===Microscope #2: Nikon Optiphot 200 (Bay 7)=== |
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* Trinocular: Ocular Binoc. + Camera (Simultaneous) |
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* Objectives: 5x, 10x, 20x, 50x, 100x, 150x |
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* Filters: Green, ND |
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* Bright/Dark Field |
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* Differential Interference Contrast (DIC/Nomarski) |
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** DIC only available on 100x and 150x mags due to image quality reduction at low mags due to DIC prisms which were removed for low mags. |
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* Top Reflected Illumination (Episcopic) |
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* AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here]. |
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*Trinocular: Ocular Binoc. + Camera (Simultaneous) |
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=== Microscope #3: Nikon Eclipse L200 (Bay 6) === |
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*Objectives: 5x, 10x, 20x, 50x, 100x, 150x |
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* [https://www.nanotech.ucsb.edu/wiki/images/8/80/Nikon-Microscope-Manual.pdf Manual for Nikon Eclipse L200D] |
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*Filters: Green, ND |
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* Trinocular: Binoc. + Camera (Mutually Exclusive) |
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*Bright/Dark Field |
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* Objectives: 5x, 10x, 20x, 50x, 100x, 150x |
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*Differential Interference Contrast (DIC/Nomarski) |
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* Filters: Green, ND |
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**DIC only available on 100x and 150x mags due to image quality reduction at low mags due to DIC prisms which were removed for low mags. |
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* Bright/Dark Field |
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*Top Reflected Illumination (Episcopic) |
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* Differential Interference Contrast (DIC/Nomarski) |
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*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here]. |
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* Top Reflected Illumination (Episcopic) |
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* AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here]. |
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=== |
===Microscope #3: Nikon Eclipse L200 (Bay 6)=== |
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* Trinocular: Binoc. + Camera (Mutually Exclusive) |
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*[https://www.nanotech.ucsb.edu/wiki/images/8/80/Nikon-Microscope-Manual.pdf Manual for Nikon Eclipse L200D] |
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* Objectives: 5x, 10x, 20x, 50x, 100x, 150x |
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*Trinocular: Binoc. + Camera (Mutually Exclusive) |
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* Filters: Green, ND |
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*Objectives: 5x, 10x, 20x, 50x, 100x, 150x |
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* Bright/Dark Field |
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*Filters: Green, ND |
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* Differential Interference Contrast (DIC/Nomarski) |
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*Bright/Dark Field |
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* Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination |
|||
*Differential Interference Contrast (DIC/Nomarski) |
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* AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here]. |
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*Top Reflected Illumination (Episcopic) |
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*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here]. |
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===Microscope #4: Nikon Eclipse L200D (Bay 6)=== |
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*Trinocular: Binoc. + Camera (Mutually Exclusive) |
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*Objectives: 5x, 10x, 20x, 50x, 100x, 150x |
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*Filters: Green, ND |
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*Bright/Dark Field |
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*Differential Interference Contrast (DIC/Nomarski) |
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*Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination |
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*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here]. |
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==Olympus== |
==Olympus== |
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The lab has two general purpose Olympus microscopes, and two specialized microscopes that require training to use, although both of these can also be used for general imaging. |
The lab has two general purpose Olympus microscopes, and two specialized microscopes that require training to use, although both of these can also be used for general imaging. |
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=== |
===[[Fluorescence Microscope (Olympus MX51)|Fluorescence Microscope: Olympus MX51 (Bay 6)]]=== |
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* ''To be added'' |
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*See the wiki page for the [[Fluorescence Microscope (Olympus MX51)|Olympus MX51]] for full details |
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*Trinocular: Binoc. + Camera (Simultaneous) |
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* See the wiki page for the [[Fluorescence Microscope (Olympus MX51)|Olympus MX51]] for full details |
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*Native Olympus Stream Software: |
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* Trinocular: Binoc. + Camera (Simultaneous) |
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**Photo/video capture |
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* Native Olympus Stream Software: |
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**Calibrated measurement (calibrations locked) |
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** Photo/video capture |
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*Objectives: 5x, 10x, 20x, 50x, 100x, 150x |
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** Calibrated measurement (calibrations locked) |
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*Filters: Green, ND |
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* Objectives: 5x, 10x, 20x, 50x, 100x, 150x |
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*Bright/Dark Field |
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* Filters: Green, ND |
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*Differential Interference Contrast (DIC/Nomarski) |
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* Bright/Dark Field |
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*Top (Episcopic) & Bottom (Diascopic) Illumination |
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* Differential Interference Contrast (DIC/Nomarski) |
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*Three Fluorescence Filters (requires training, see main tool page for specs.) |
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* Top (Episcopic) & Bottom (Diascopic) Illumination |
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* Three Fluorescence Filters (requires training, see main tool page for specs.) |
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=== |
===[[Deep UV Optical Microscope (Olympus)|DUV Microscope: Olympus MX61A-DUV (Bay 7)]]=== |
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Please see the main tool page for detailed info on this microscope: [[Deep UV Optical Microscope (Olympus)]] |
Please see the main tool page for detailed info on this microscope: [[Deep UV Optical Microscope (Olympus)]] |
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YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training. |
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training. |
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* Motorized Stage + Objective Turret |
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* Trinocular: Binoc. + Camera (Simultaneous) |
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* Objectives: 5x, 10x, 20, 50x, 100x, DUV-100x |
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* Filters: ''to be added'' |
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* Native Olympus MX61 Software control & Camera |
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** Calibrated measurements (calibrations locked) |
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** Z (focus) measurement via motorized stage height |
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* Deep-UV Light source + DUV Camera |
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** DUV-100x sub-micron imaging/measurement |
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*Motorized Stage + Objective Turret |
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== [[Laser Scanning Confocal M-scope (Olympus LEXT)|Olympus LEXT Confocal Microscope (Bay 4)]] == |
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*Trinocular: Binoc. + Camera (Simultaneous) |
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*Objectives: 5x, 10x, 20, 50x, 100x, DUV-100x |
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*Filters: ''to be added'' |
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*Native Olympus MX61 Software control & Camera |
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**Calibrated measurements (calibrations locked) |
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**Z (focus) measurement via motorized stage height |
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*Deep-UV Light source + DUV Camera |
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**DUV-100x sub-micron imaging/measurement |
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==[[Laser Scanning Confocal M-scope (Olympus LEXT)|Olympus LEXT Confocal Microscope (Bay 4)]]== |
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See the main tool page for complete info: [[Laser Scanning Confocal M-scope (Olympus LEXT)]] |
See the main tool page for complete info: [[Laser Scanning Confocal M-scope (Olympus LEXT)]] |
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YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training. |
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training. |
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* Motorized stage + Objective Turret |
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*Motorized stage + Objective Turret |
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* 100mm wafer stage |
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*100mm wafer stage |
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* Native Olympus OLS2000 Software & Built-In Camera: |
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*Native Olympus OLS2000 Software & Built-In Camera: |
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** Calibrated measurement (calibrations locked) |
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**Calibrated measurement (calibrations locked) |
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** Image stitching capabilities |
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**Image stitching capabilities |
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* 3D Laser-Scanning Confocal Microscopy capability: |
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*3D Laser-Scanning Confocal Microscopy capability: |
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** 3D Topographical measurement (optical profilometry) |
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**3D Topographical measurement (optical profilometry) |
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** Surface roughness estimations (large roughness) |
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**Surface roughness estimations (large roughness) |
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** Thin-Film Film-Thickness Measurements (thicker films) |
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**Thin-Film Film-Thickness Measurements (thicker films) |
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==Documentation== |
==Documentation== |
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* [[UCSB NanoFab Microscope Training|Microscope Training]] - General procedures and info for using our microscopes |
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*[[UCSB NanoFab Microscope Training|'''Microscope Training''']] - General procedures and info for using our microscopes |
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*[[media:Nikon-Microscope-Manual.pdf|Nikon Microscope Instruction Manual (Ecplise)]] |
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*[[media:Nikon-Microscope-OptiPhot-Manual.pdf|Nikon Microscope Instruction Manual (Optiphot)]] |
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*[//www.nanotech.ucsb.edu/wiki/images/8/80/Nikon-Microscope-Manual.pdf Nikon Microscope Instruction Manual (Ecplise)] |
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*[//www.nanotech.ucsb.edu/wiki/images/2/28/Nikon-Microscope-OptiPhot-Manual.pdf Nikon Microscope Instruction Manual (Optiphot)] |
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*[https://www.amscope.com/software/AmScope/MU-Series-Complete-Manual-Complete.pdf AM Scope manual link]. |
*[https://www.amscope.com/software/AmScope/MU-Series-Complete-Manual-Complete.pdf AM Scope manual link]. |
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*[https://www.amscope.com/software-download AM Scope Software link]. |
*[https://www.amscope.com/software-download AM Scope Software link]. |
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Revision as of 17:53, 25 September 2019
|
Microscope Training - Use this guide for general usage info & the many advanced features available on most of our microscopes.
Nikon
The Nikon scopes have 3rd party cameras that are connected to computers via AM Scope software which allows users to digitally capture images through the microscope and make calibrated measurements.
Microscope #1: Nikon Optiphot-2 (Char. Lab, Room 1111)
- Trinocular: Ocular Binoc. + Camera (Exclusive)
- LED Illuminator, Variable
- Objectives: Info to be added
- Bright/Dark Field
- Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
- AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.
- User Manual: Nikon Optiphot-2 (PDF)
Microscope #2: Nikon Optiphot 200 (Bay 7)
- Trinocular: Ocular Binoc. + Camera (Simultaneous)
- Objectives: 5x, 10x, 20x, 50x, 100x, 150x
- Filters: Green, ND
- Bright/Dark Field
- Differential Interference Contrast (DIC/Nomarski)
- DIC only available on 100x and 150x mags due to image quality reduction at low mags due to DIC prisms which were removed for low mags.
- Top Reflected Illumination (Episcopic)
- AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.
Microscope #3: Nikon Eclipse L200 (Bay 6)
- Manual for Nikon Eclipse L200D
- Trinocular: Binoc. + Camera (Mutually Exclusive)
- Objectives: 5x, 10x, 20x, 50x, 100x, 150x
- Filters: Green, ND
- Bright/Dark Field
- Differential Interference Contrast (DIC/Nomarski)
- Top Reflected Illumination (Episcopic)
- AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.
Microscope #4: Nikon Eclipse L200D (Bay 6)
- Trinocular: Binoc. + Camera (Mutually Exclusive)
- Objectives: 5x, 10x, 20x, 50x, 100x, 150x
- Filters: Green, ND
- Bright/Dark Field
- Differential Interference Contrast (DIC/Nomarski)
- Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
- AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.
Olympus
The lab has two general purpose Olympus microscopes, and two specialized microscopes that require training to use, although both of these can also be used for general imaging.
Fluorescence Microscope: Olympus MX51 (Bay 6)
- See the wiki page for the Olympus MX51 for full details
- Trinocular: Binoc. + Camera (Simultaneous)
- Native Olympus Stream Software:
- Photo/video capture
- Calibrated measurement (calibrations locked)
- Objectives: 5x, 10x, 20x, 50x, 100x, 150x
- Filters: Green, ND
- Bright/Dark Field
- Differential Interference Contrast (DIC/Nomarski)
- Top (Episcopic) & Bottom (Diascopic) Illumination
- Three Fluorescence Filters (requires training, see main tool page for specs.)
DUV Microscope: Olympus MX61A-DUV (Bay 7)
Please see the main tool page for detailed info on this microscope: Deep UV Optical Microscope (Olympus)
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
- Motorized Stage + Objective Turret
- Trinocular: Binoc. + Camera (Simultaneous)
- Objectives: 5x, 10x, 20, 50x, 100x, DUV-100x
- Filters: to be added
- Native Olympus MX61 Software control & Camera
- Calibrated measurements (calibrations locked)
- Z (focus) measurement via motorized stage height
- Deep-UV Light source + DUV Camera
- DUV-100x sub-micron imaging/measurement
Olympus LEXT Confocal Microscope (Bay 4)
See the main tool page for complete info: Laser Scanning Confocal M-scope (Olympus LEXT)
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
- Motorized stage + Objective Turret
- 100mm wafer stage
- Native Olympus OLS2000 Software & Built-In Camera:
- Calibrated measurement (calibrations locked)
- Image stitching capabilities
- 3D Laser-Scanning Confocal Microscopy capability:
- 3D Topographical measurement (optical profilometry)
- Surface roughness estimations (large roughness)
- Thin-Film Film-Thickness Measurements (thicker films)
Documentation
- Microscope Training - General procedures and info for using our microscopes